Akt Increases Sarcoplasmic Reticulum Ca2+ Cycling by Direct Phosphorylation of Phospholamban at Thr17

Wild-type mice, transgenic mice overexpressing active Akt (Akt-E40K), mice with inducible cardiac-specific knock-out of phosphatidylinositol-dependent kinase-1, and in vitro cardiomyocytes

Overexpression of active Akt (Akt-E40K) or SR-targeted Akt, and knock-out of phosphatidylinositol-dependent kinase-1

Wild-type mice and control cardiomyocytes

Sarcoplasmic reticulum (SR) Ca2+ uptake and phosphorylation status of phospholamban (PLN) at Thr17surrogate