The distribution of NOS expression within infarcted myocardium in humans is comparable to experimental animal models, with iNOS localizing to infiltrating macrophages and non-necrotic cardiomyocytes.
Supports human-animal model concordance for post-MI NOS expression; hypothesis-generating with no immediate clinical implications.
The present study is concerned with the expression of inducible (iNOS) and constutive nitric oxide synthase (cNOS) in human myocardial infarction. This investigation is based on previous studies which showed a time related activation of iNOS derived from infiltrating macrophages in infarcted rabbit myocardium. Specific monoclonal antibodies to both human isoforms were used in order to localize iNOS and cNOS in tissue sections from 6 patients who died on days 2, 7 and 25 after myocardial infarction. In addition, infiltrating macrophages were identified using a monoclonal antibody against human macrophages (CD68). Results showed that, comparable to the animal model, iNOS was primarily located in macrophages infiltrating the infarcted region; this was observed at days 7 and 25 after clinical diagnosis. iNOS was also found in non-necrotic cardiomyocytes within or close to the infarcted region and closely associated with the intercalated discs (IC). In all infarcts, cNOS was expressed in endothelial cells of preserved vasculature and in endocardial cells. It is concluded that the distribution of NOS expression within infarcted myocardium in humans is comparable to the findings in the experimental animal model.
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Wildhirt et al. (1995) studied this question.
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