March 15, 1996Circulation
Expression of Inducible Nitric Oxide Synthase in Human Heart Failure
Key result
Inducible nitric oxide synthase (iNOS) expression was detected in 71% of ventricular myocardium biopsies from patients with heart failure compared to 0% in control subjects (P<.0001).
Authors
Discussion
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Implication
iNOS expression in failing myocardium is hypothesis-generating; leaves open therapeutic targeting in heart failure.
Key Points
- This study aims to explore the role of inducible nitric oxide synthase (iNOS) in human heart failure.
- Analyzed iNOS expression in myocardial samples from control subjects, donor hearts, and heart failure patients.
- Used reverse transcription-polymerase chain reaction to detect iNOS and atrial natriuretic peptide (ANP).
- Performed immunohistochemistry and Western blot analysis on hearts to evaluate iNOS protein expression.
- iNOS expressed in 71% of heart failure biopsies compared to none in controls (P<.0001).
- 50% of donor hearts exhibited iNOS expression, with varying rates across heart failure types: 67% DCM, 59% IHD, and 100% VHD.
- Positive iNOS protein staining was seen in all failing hearts, while donor hearts showed minimal expression.
Study Design
Type
Observational (n=72)
PICO
P
Population
Heart failure (n=72)
O
Primary Outcome
iNOS expression in ventricular myocardium, p=<.0001
Main Result
Absolute Event Rate: 71% vs 0%
p-value: p=<.0001
Cite This Study
Haywood et al. (1996) conducted an observational in Heart failure (n=72). Inducible nitric oxide synthase (iNOS) expression was detected in 71% of ventricular myocardium biopsies from patients with heart failure compared to 0% in control subjects (P<.0001).