Answers are grounded in this paper and cite their sources.
A rapid method for localizing enzymatic levels in various structures of the kidney is described. This method consists of dissecting a cone from rapidly frozen kidney, and measuring the enzyme activities of thin slices from the cortex to the papilla. The use of this method in elucidating the distribution of d- and l-amino acid oxidases and of hexokinase and glucose 6-phosphatase in adult rat kidney and of the last two enzymes in young rat kidney from birth to weaning is reported. Glucose 6-phosphatase was most active in the cortex and least active in the papilla in all age groups. The site of maximum glucose 6-phosphatase activity coincides with the alleged site of glucose reabsorption. Hexokinase was highest in the papilla and lowest in the outer cortex in most ages. l- Amino acid oxidase was concentrated in the midcortex, and d-amino acid oxidase in the border between the cortex and medulla.
No takes yet. Share an insight, caveat, or question.
Waldman et al. (1963) studied this question.
Synapse has enriched 10 related papers. Consider them for comparative context: