KEPHALINS 3 turbid again on removing the ammonia by dialysis. A translucent emulsion of fraction I also becomes transparent when ammonia was added, but it soon became very turbid again, and on standing a white precipitate formed with a clear supernatant which became turbid on dialysis. The white precipitate contained inorganic phosphate and lipids. SUMMARY 1. When a turbid water emulsion of inositol phosphatide fraction (Folch's fraction I) or phosphatidylserine (Folch's fraction III) from brain tissues is added to a solution of ethylenediaminetetra-acetate at pH 10, the solution quickly becomes transparent and remains so even after removal by dialysis of the reagents added. Such a phenomenon is not seen with phosphatidylethanolamine (Folch's fraction V) or with a brain- 'lecithin' fraction.
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Kimura et al. (1960) studied this question.
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