Strelitzia reginae Leaves: Phytochemical Elucidation and Antimicrobial Assessment on Clinical Isolates and Standard Strains

Strelitzia reginae is used in some African countries as a traditional medicine with very fewphytochemical and biological assessments. The study aims to isolate and structure elucidate of S. reginaephytoconstituents using different chromatographic and spectral techniques. In addition to, antimicrobialassessment of ethyl acetate and n-butanol fractions on both clinical isolates and standard strains. Strelitziareginae belongs to the family Strelitziaceae which is a rich source of biologically active phytoconstituents andhas diverse therapeutic values. Strelitzia reginae leaves were fractionated into ethyl acetate and n-butanol, andfive compounds were detected. Compounds were identified as kaempferol (1), quercetin (2), daucosterol (3),vitexin (4), and rutin (5). The isolated compounds were elucidated by their NMR and ESI-MS spectral data.Antimicrobial activity of Strelitzia reginae ethyl acetate and n-butanol fractions screened against six differentbacterial strains (three Gram-positive, three Gram-negative, and one fungus isolate). Both fractions showedantimicrobial activities against Klebsiella pneumonia and Staphylococcus aureus which is methicillinresistant (MRSA) strains. As well, the activity of antimicrobial was detected against Proteus mirabilis clinicalisolate from urine and MRSA. On the other hand, both fractions showed inadequate antimicrobial activitiesagainst the Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans clinical isolates comparedto reference drugs. The antimicrobial results were in accordance with previously reported folk uses andattributed to the isolated phytoconstituents.