April 15, 1997Journal of NeuroscienceOpen Access

Identification of the Single Channels that Underlie the N-Type and L-Type Calcium Currents in Bullfrog Sympathetic Neurons

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Population

Neurons dissociated from caudal paravertebral sympathetic ganglia of adult bullfrogs (Rana catesbeiana)

Comparison

omega-conotoxin GVIA and Bay K 8644 applied to… vs Patches not exposed to omegaCGVIA or before…

Design

Preclinical

Authors

KEKeith S. ElmslieA.T. Still University

Discussion

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Implication

Clarifies N-type channel identity in sympathetic neurons; leaves open therapeutic targeting in cardiovascular autonomic disorders.

Structured PICO

Population

Neurons dissociated from caudal paravertebral sympathetic ganglia of adult bullfrogs (Rana catesbeiana)

Intervention

omega-conotoxin GVIA (omegaCGVIA) and Bay K 8644 applied to cell-attached patches

Comparator

Patches not exposed to omegaCGVIA or before application

Outcome

Single-channel conductance, current amplitude, voltage activation range, and sensitivity to omegaCGVIA and Bay K 8644surrogate

This study successfully identifies the single-channel correlate (20 pS conductance) of the macroscopic N-type calcium current in sympathetic neurons, resolving previous misidentifications.

Limitations

Absence of single-Ef-channel patches prevented distinguishing among multiple channel types, subconductance states, and/or different gating modes for Ef-channels.

Cite This Study

Keith S. Elmslie (1997) studied this question.

synapsesocial.com/papers/6a90104733cf5c591c0d35bf https://doi.org/10.1523/jneurosci.17-08-02658.1997

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Identification of the Single Channels that Underlie the N-Type and L-Type Calcium Currents in Bullfrog Sympathetic Neurons

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Identification of the Single Channels that Underlie the N-Type and L-Type Calcium Currents in Bullfrog Sympathetic Neurons

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Also Consider

Also Consider