January 1, 1993Nucleic Acids ResearchOpen Access

Secondary structure of the HIV-2 leader RNA comprising the tRNA-primer binding site

View Full Paper

Ask AI

Bookmark

Population

In vitro synthesized HIV-2 leader RNA and nucleotide sequences from 13 retroviral isolates

Design

Preclinical

Authors

BBBen BerkhoutAmsterdam University Medical Centers ISIlse SchoneveldUniversity of Amsterdam

Discussion

Member takes

Implication

May enhance HIV-2 tRNA priming; leaves open conservation and antiviral targeting in future models.

Structured PICO

Population

In vitro synthesized HIV-2 leader RNA (from plasmids Blue-TAR2 and Blue-Gag2) and nucleotide sequences from 13 retroviral isolates (7 HIV-2, 6 SIV)

Intervention

Chemical and enzymatic structure probing (RNase T1, cobra venom nuclease, nuclease S1, diethyl-pyrocarbonate, dimethylsulfate, kethoxal) combined with phylogenetic comparative sequence analysis

Outcome

Secondary structure model of the HIV-2 leader RNA comprising the tRNA-primer binding site (PBS)

The secondary structure of the HIV-2 leader RNA reveals that the primer-binding site is largely exposed in an internal loop, which likely facilitates optimal interaction with the tRNA primer for the initiation of reverse transcription.

Cite This Study

Berkhout et al. (1993) studied this question.

synapsesocial.com/papers/6a901f60f009383eee840ad3 https://doi.org/10.1093/nar/21.5.1171

View Full Paper

Ask AI

Bookmark

Secondary structure of the HIV-2 leader RNA comprising the tRNA-primer binding site

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Secondary structure of the HIV-2 leader RNA comprising the tRNA-primer binding site

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider