Key result
Calcific aortic valve disease was associated with 92 differentially expressed miRNAs compared to healthy controls, categorizing samples into two well-defined clusters.
Why the study?
What is the microRNA expression signature in human calcific aortic valve disease compared to healthy controls?
Case-Control (n=8)
What is the microRNA expression signature in human calcific aortic valve disease compared to healthy controls?
The identification of 92 aberrantly expressed miRNAs in calcific aortic valve disease provides a specific molecular signature and potential novel therapeutic targets.
Hypothesis-generating for miRNA signatures in calcific aortic valve disease; prospective studies required before clinical consideration.
Altered microRNA (miRNA, miR) expression has been related to many disease processes; however, the miRNA expression signature in calcific aortic valve disease (CAVD) is unclear. In this study, microarrays were used to determine the miRNA expression signature of tissue samples from healthy individuals ( n=4 ) and patients with CAVD ( n=4 ). TargetScan, PITA, and microRNAorg 3-way databases were used to predict the potential target genes. DIANA-miRPath was used to incorporate the aberrant miRNAs into gene pathways. miRNA microarrays identified 92 differentially expressed miRNAs in CAVD tissues. The principal component analysis (PCA) of these samples and the unsupervised hierarchical clustering analysis based on the 92 aberrantly expressed miRNAs noted that miRNA expression could be categorized into two well-defined clusters that corresponded to healthy control and CAVD. Bioinformatic analysis showed the miRNA targets and potential molecular pathways. Collectively, our study reported the miRNA expression signature in CAVD and may provide potential therapeutic targets for CAVD.
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Wang et al. (2017) conducted a case-control in Calcific aortic valve disease (n=8). Calcific aortic valve disease vs. Healthy individuals was evaluated on Differentially expressed miRNAs. Calcific aortic valve disease was associated with 92 differentially expressed miRNAs compared to healthy controls, categorizing samples into two well-defined clusters.
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