Detection of Diazepam and Determination of Time of Ingestion

Levels of diazepam metabolites excreted in the urine of eight male subjects were measured for several weeks after ingestion of a single therapeutic dose of 10 mg. Analyses were carried out using gas chromatography-mass spectrometric (GC/MS) procedures. Ratios of oxazepam to desmethyldiazepam and oxazepam to temazepam below 1.0 and 0.7, respectively, were characteristic of consumption within the previous 24 hours. Estimations of dosage and time since ingestion were made on samples from four Individuals who had ingested 5 mg diazepam by comparing urinary metabolite concentrations and ratios to the control group data.