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Elucidate the evolutionary origins of vertebrate recombination-activating genes by cloning RAG2 and characterizing the genomic RAG1/RAG2 locus in the sandbar shark.

January 22, 2003The FASEB Journal

Cloning of shark RAG2 and characterization of the RAG1/RAG2 gene locus

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Authors

SSSamuel F. SchluterUniversity of Arizona JMJohn J. MarchalonisUniversity of North Carolina at Chapel Hill

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Implication

Comparative genomic analysis uncovers structural conservation of the RAG1/RAG2 locus in sandbar sharks, indicating ancient origins of vertebrate adaptive immunity.

Key Points

Elucidate the evolutionary origins of vertebrate recombination-activating genes by cloning RAG2 and characterizing the genomic RAG1/RAG2 locus in the sandbar shark.
Cloned and sequenced the RAG2 gene from the sandbar shark (Carcharhinus plumbeus).
Characterized the genomic architecture, orientation, and intergenic spacing of the shark RAG1/RAG2 gene locus.
Identified a 520-amino-acid shark RAG2 protein that shares approximately 50% sequence identity with other vertebrate homologs and retains three conserved core functional domains.
Demonstrated that shark RAG2 lacks introns and lies adjacent to RAG1 in an opposite transcriptional orientation.
Resolved a 9.4-kb intergenic region between RAG1 and RAG2 that is larger than in teleosts (2-3 kb), comparable to tetrapods, and contains SINE and LINE retrotransposon fragments.

Cite This Study

Schluter et al. (2003) studied this question.

synapsesocial.com/papers/6a9047788ecb2c271c9095f9 https://doi.org/10.1096/fj.02-0565fje

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Implication

Comparative genomic analysis uncovers structural conservation of the RAG1/RAG2 locus in sandbar sharks, indicating ancient origins of vertebrate adaptive immunity.

Cloning of shark RAG2 and characterization of the RAG1/RAG2 gene locus

Discussion

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Implication

Key Points

Cite This Study

Cloning of shark RAG2 and characterization of the RAG1/RAG2 gene locus

Discussion

Member takes

Implication

Key Points

Cite This Study

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