What question did this study set out to answer?

To identify the intracellular signaling mechanisms and functional immune responses triggered by CD46 ligation in maturing human macrophages.

November 1, 2000The Journal of Immunology

Functional Modulation of Human Macrophages Through CD46 (Measles Virus Receptor): Production of IL-12 p40 and Nitric Oxide in Association with Recruitment of Protein-Tyrosine Phosphatase SHP-1 to CD46

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Authors

MKMitsue Kurita-TaniguchiOsaka International Cancer Institute AFAya FukuiNagoya University KHKaoru HazekiHiroshima University

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Implication

In vitro study demonstrates CD46 ligation triggers IL-12 p40 and nitric oxide production via SHP-1 recruitment in macrophages, indicating ligand-dependent immune signaling.

Key Points

To identify the intracellular signaling mechanisms and functional immune responses triggered by CD46 ligation in maturing human macrophages.
Cultured human peripheral blood monocytes with GM-CSF for 6 to 8 days to induce macrophage differentiation.
Stimulated differentiated macrophages with anti-CD46 F(ab')2 monoclonal antibody fragments or the wild-type measles virus Kohno (KO) strain (UV-inactivated or live).
Evaluated IL-12 p40 and nitric oxide production, viral replication, and the recruitment dynamics of protein-tyrosine phosphatase SHP-1 to CD46.
Direct CD46 ligation by antibody fragments or the KO strain induced IL-12 p40 and nitric oxide synthesis in GM-CSF-differentiated macrophages without requiring intracellular viral replication.
Stimulation with the KO strain triggered rapid, transient dissociation of SHP-1 from CD46 in mature macrophages.
The wild-type KO strain failed to replicate in 6- to 8-day GM-CSF-cultured macrophages, in contrast to other measles virus strains that replicated and formed syncytia.

Cite This Study

Kurita-Taniguchi et al. (2000) studied this question.

synapsesocial.com/papers/6a904d64c63bb9fb07dd8545 https://doi.org/10.4049/jimmunol.165.9.5143

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Implication

In vitro study demonstrates CD46 ligation triggers IL-12 p40 and nitric oxide production via SHP-1 recruitment in macrophages, indicating ligand-dependent immune signaling.

Functional Modulation of Human Macrophages Through CD46 (Measles Virus Receptor): Production of IL-12 p40 and Nitric Oxide in Association with Recruitment of Protein-Tyrosine Phosphatase SHP-1 to CD46

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Key Points

Cite This Study

Functional Modulation of Human Macrophages Through CD46 (Measles Virus Receptor): Production of IL-12 p40 and Nitric Oxide in Association with Recruitment of Protein-Tyrosine Phosphatase SHP-1 to CD46

Discussion

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Implication

Key Points

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