What question did this study set out to answer?

Determine the effects of phospholipase C treatment and cell fusion inhibition on phospholipid turnover and synthesis in embryonic myogenic cells.

September 1, 1979Proceedings of the National Academy of SciencesOpen Access

Stimulation of phospholipid metabolism in embryonic muscle cells treated with phospholipase C.

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Authors

CKC KentUniversity of Pittsburgh

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Implication

In vitro study reveals accelerated phospholipid synthesis in embryonic muscle cells treated with phospholipase C, suggesting a homeostatic feedback mechanism regulating membrane composition.

Key Points

Determine the effects of phospholipase C treatment and cell fusion inhibition on phospholipid turnover and synthesis in embryonic myogenic cells.
Cultured embryonic myogenic cells were treated with phospholipase C (phosphatidylcholine choline-phosphohydrolase) to degrade exogenous phosphatidylcholine and inhibit cell fusion.
Quantified phospholipid synthesis and degradation rates by measuring 32Pi and [methyl-3H]choline incorporation alongside the release of [32P]phosphocholine into culture medium relative to untreated fusing cells and calcium-deprived non-fusing cells.
Incorporation rates of 32Pi and [methyl-3H]choline were approximately 5-fold greater in phospholipase C-treated cells than in untreated fusing cells or calcium-deprived control cells.
Synthesis was selectively enhanced for phosphatidylcholine and sphingomyelin, whereas synthesis of cardiolipin and phosphatidylinositol showed no stimulation.
Total cellular phospholipid levels and individual phospholipid class concentrations remained steady, balanced by accelerated degradation of cellular [32P]phosphatidylcholine into extracellular [32P]phosphocholine.

Cite This Study

C Kent (1979) studied this question.

synapsesocial.com/papers/6a905445e73a4c404fc933fc https://doi.org/10.1073/pnas.76.9.4474

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September 1, 1979Proceedings of the National Academy of SciencesOpen Access

Stimulation of phospholipid metabolism in embryonic muscle cells treated with phospholipase C.

View Full Paper

Ask AI

Bookmark

Authors

CKC KentUniversity of Pittsburgh

Discussion

Member takes

Implication

In vitro study reveals accelerated phospholipid synthesis in embryonic muscle cells treated with phospholipase C, suggesting a homeostatic feedback mechanism regulating membrane composition.

Key Points

Determine the effects of phospholipase C treatment and cell fusion inhibition on phospholipid turnover and synthesis in embryonic myogenic cells.
Cultured embryonic myogenic cells were treated with phospholipase C (phosphatidylcholine choline-phosphohydrolase) to degrade exogenous phosphatidylcholine and inhibit cell fusion.
Quantified phospholipid synthesis and degradation rates by measuring 32Pi and [methyl-3H]choline incorporation alongside the release of [32P]phosphocholine into culture medium relative to untreated fusing cells and calcium-deprived non-fusing cells.
Incorporation rates of 32Pi and [methyl-3H]choline were approximately 5-fold greater in phospholipase C-treated cells than in untreated fusing cells or calcium-deprived control cells.
Synthesis was selectively enhanced for phosphatidylcholine and sphingomyelin, whereas synthesis of cardiolipin and phosphatidylinositol showed no stimulation.
Total cellular phospholipid levels and individual phospholipid class concentrations remained steady, balanced by accelerated degradation of cellular [32P]phosphatidylcholine into extracellular [32P]phosphocholine.

Cite This Study

C Kent (1979) studied this question.

synapsesocial.com/papers/6a905445e73a4c404fc933fc https://doi.org/10.1073/pnas.76.9.4474

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