Primary Site of Coxsackievirus B Replication in the Small Intestines: No Proof of Peyer’s Patches Involvement

Figures

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  Figure 1. Peyer’s patches (PP). (a) Hematoxylin eosin staining of a typical PPs with enlarged germinating centers at day 7 post infection (p.i.) of orally infected mouse with CVB3-Nancy, Set 1 (20×); (b) normal PP of the mock infected control mouse at day 7 p.i. of an orally infected mouse, Set 1 (20×); (c) Localization of IFN-α in the PP, the brown staining, by immunohistochemical analysis (40×), Set (I) at day 3 p.i. (40×); (d) Small intestine sample of mock infected control mouse from Set I at day 3 p.i. (20×).

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  Figure 2. Localization of viral markers in mouse SI tissue sections after CVB infection by immunohistochemical analysis and in situ hybridization. (a) VP1 staining of the SI by method 1 of mock infected control (PBS) (40×); (b) SI of orally infected mouse (CVB4-JVB) of Set IIa mice, 4 h p.i. by method 1 (40×); (c) tissue section of SI of Set I (CVB3) infected mice stained by using method 2, SI of orally infected day 7 p.i. Arrows show the positivity in the FAE region (40×); (d) SI of i.p. infected mouse, day 7 p.i. (40×) (e) in situ hybridization of the SI tissue of the control (PBS) (40×), day 3 p.i. after oral infection; (f) orally (CVB3 infection) infected mouse of Set I mice 3 days p.i. (60×).

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  Figure 3. Localization of eGFP-CVB3 in the small intestines of infected mice. The fluorescent signal of enhanced green fluorescent protein (eGFP) can be seen as bright yellowish green fluorescent. The bright yellow arrows show the positive signals of the eGFP positive infected areas. (a) absence of fluorescence at 4h in the mock infected control mice, 20×; (b) At 6 h p.i. fluorescence can be seen in the sub-epithelial dome area of the PP and the FAE area after oral infection 20× with zoomed in 40×; (c) At 12 h p.i. fluorescence can be seen in the dome area of the PP and the FAE area after i.p. infection 20×; (d) at 8 h p.i. eGFP can be seen in the base of the villi or crypts of Lieberkuhn 20× and zoomed in at 40×. The yellow arrows show positive signals.