Why the study?
Does reverse transcription-polymerase chain reaction accurately classify respiratory tract picornavirus isolates compared to acid lability testing?
Does reverse transcription-polymerase chain reaction accurately classify respiratory tract picornavirus isolates compared to acid lability testing?
RT-PCR is an effective alternative to traditional acid lability testing for differentiating human rhinoviruses from enteroviruses.
Supports RT-PCR as alternative to acid lability for HRV/EV differentiation; leaves open validation in larger clinical cohorts.
The classification of picornaviruses isolated from respiratory secretions as human rhinoviruses (HRVs) or enteroviruses (EVs) by using reverse transcription-polymerase chain reaction was compared to that derived from acid lability testing. Of the 135 clinical isolates examined, 91 were found to be HRVs and 44 were EVs. There was 100% concordance between the two classification methods. Reverse transcription-polymerase chain reaction is an effective alternative to traditional methods for differentiating HRVs from EVs.
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Atmar et al. (1993) studied this question.
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