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June 1, 1985Journal of Medical Virology

Detection of enteric adenoviruses by dot‐blot hybridization using a molecularly cloned viral DNA probe

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Why the study?

Does a dot-blot hybridization assay accurately detect enteric adenoviruses compared to endonuclease analysis in stool samples and tissue culture lysates?

Population

Stool samples and tissue culture lysates prescreened by electron microscopy, cell culture or ELISA

Comparison

Dot-blot hybridization assay for enteric… vs Endonuclease analysis

Design

Other, Tested in a blind fashion

Authors

HTHoward TakiffInstituto Venezolano de Investigaciones CientíficasMSMindell SeidlinChildren's Tumor FoundationPKPhilip R. KrauseCenter for Biologics Evaluation and Research

Discussion

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Implication

May support rapid EAd detection research; leaves open clinical utility pending larger prospective validation.

Structured PICO

Does a dot-blot hybridization assay accurately detect enteric adenoviruses compared to endonuclease analysis in stool samples and tissue culture lysates?

P
Population
Stool samples and tissue culture lysates prescreened by electron microscopy, cell culture or ELISA
I
Intervention
Dot-blot hybridization assay for enteric adenovirus (EAd) DNA using a cloned DNA fragment
C
Comparator
Endonuclease analysis (standard for typing isolates)
O
Outcome
Sensitivity and specificity for detecting EAds and distinguishing them from other adenovirusessurrogate

A newly developed dot-blot hybridization assay demonstrates 91% sensitivity and 71% specificity for detecting enteric adenoviruses, offering a potential rapid diagnostic tool.

Limitations

  • False-positive and equivocal dot-blot results appeared to be caused by other Ads.

Cite This Study

Takiff et al. (1985) studied this question.

synapsesocial.com/papers/6a90743cccd91641af4dd990https://doi.org/10.1002/jmv.1890160203
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Also Consider

Synapse has enriched 4 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Diagnosis of fastidious enteric adenoviruses 40 and 41 in stool specimens1984 · 75 citations
  2. 2Specific properties of two enteric adenovirus 41 clones mapped within early region 1A1985 · 19 citations
  3. 3Cloning and physical mapping of enteric adenoviruses (candidate types 40 and 41)1984 · 33 citations
  4. 4Rapid and sensitive colorimetric method for visualizing biotin-labeled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: Bio-blots.1983 · 1,065 citations