Metabolism of Bovine Semen. IX. Glutamic-Oxaloacetic and Glutamic-Pyruvic Transaminase Activities

Summary Assays on 63 samples of bovine semen gave an average of 623±48 units of glutamic-oxaloacetic transaminase (GO-T) and 15±1 units of glutamic-pyruvic transaminase (GP-T) per milliliter of seminal plasma. Statistically significant correlations of .849 for GO-T and .409 for GP-T were observed between seminal plasma transaminase activity and numbers of spermatozoa per milliliter of semen. When spermatozoa were freed of seminal plasma and the GO-T and GP-T activities in seminal plasma and cells compared, more activity was associated with the cells than with seminal plasma. With seminal plasma-free cells, GO-T per milligram of dry weight was similar to that reported in several bovine tissues, but lower than the activity in heart, liver, and skeletal muscle.