February 1, 1987Journal of NeuroscienceOpen Access

Solubilization of the apamin receptor associated with a calcium- activated potassium channel from rat brain

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Population

Rat brain synaptic membranes from decerebellate brains of Wistar rats

Design

Preclinical

Authors

MSM J SeagarCentre National de la Recherche Scientifique BMBéatrice Marquèze‐PoueyInserm FCFrançois CouraudCentre National de la Recherche Scientifique

Discussion

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Implication

Enables SK channel purification in animal models; leaves open translation to human cardiac electrophysiology.

Structured PICO

Population

Rat brain synaptic membranes from decerebellate brains of Wistar rats

Intervention

Solubilization using sodium cholate (5% wt/vol)

Outcome

Receptor yield, stability, binding affinity (Kd), and binding capacity for 125I-apaminsurrogate

The apamin receptor, associated with a calcium-activated potassium channel, can be solubilized from rat brain while retaining its pharmacological properties and regulatory K+ binding site.

Limitations

Purification of the apamin receptor to homogeneity is difficult to envisage due to the required purification factor of 200,000.

Cite This Study

Seagar et al. (1987) studied this question.

synapsesocial.com/papers/6a90848efdb099d0d8dd8b98 https://doi.org/10.1523/jneurosci.07-02-00565.1987

View Full Paper

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Solubilization of the apamin receptor associated with a calcium- activated potassium channel from rat brain

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Solubilization of the apamin receptor associated with a calcium- activated potassium channel from rat brain

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Also Consider

Also Consider