Answers are grounded in this paper and cite their sources.
A modification of the thiocholine technique for cholinesterases is described, in which mounted sections are dehydrated for 2 min in each ascending grade of alcohol before development of CuS by using alcoholic (NH4)2S. This reagent is prepared by first saturating half-concentrated (NH4)2OH with H2S; 3 ml of this is diluted with 22 ml of ethyl alcohol, then saturated with CuS. With these modifications better definition of sites of cholinesterase activity is achieved.
No takes yet. Share an insight, caveat, or question.
Bull et al. (1957) studied this question.
Synapse has enriched 10 related papers. Consider them for comparative context: