Mechanism of translation of the hepadnaviral polymerase (P) gene.
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Population
COS-7 cells and HepG2 cells transfected with plasmids containing duck hepatitis B virus (DHBV) genes
Comparison
Transfection with plasmids containing DHBV… vs Wild-type constructs or mock-transfected cells
Design
Preclinical
Authors
Discussion
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Member takes
Implication
May inform hepadnaviral polymerase targeting; leaves open translation to human therapeutics from animal data.
Structured PICO
P
Population
COS-7 cells and HepG2 cells transfected with plasmids containing duck hepatitis B virus (DHBV) genes
I
Intervention
Transfection with plasmids containing DHBV polymerase (P) gene fused to lacZ (AP13) and various mutations
C
Comparator
Wild-type constructs (AP13, AB1) or mock-transfected cells
O
Outcome
Translational products (P-Z and C-Z fusion proteins) and mRNA transcripts
The translation of the hepadnaviral polymerase gene occurs via internal initiation from its own AUG codon on a bicistronic mRNA, independent of ribosomal scanning from the 5' end.
Cite This Study
Chang et al. (1990) studied this question.
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