A method for the estimation of ethanol and volatile fatty acids by gas chromatography by direct treatment of cultivation broth is described. The packing material was Hallcomid M‐18 with 1% H3PO4 on Chromosorb W AW. The separation was made at 132°C with nitrogen as a carrier gas; for detection a flame ionisation detector was used. The analysis of oxygen and carbon dioxide of the inlet and outlet gas from a fermentor was also studied. For the separation of the gases the combination of molecular seive 5A and Chromosorb 102 was used. The analysis was performed at 35°C with helium as a carrier gas and a thermal conductivity detector was used. With the experimental conditions found in this study the methods described gave reproducible and accurate results for the analytical control of cell cultivation processes.
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Unger et al. (1977) studied this question.
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