~4 rennet replacement enzyme complex derived from Bacillus subtilis by fermen~ tation was studied. By means of ionexchange chromatography, three proteases could be isolated from the enzyme complex. The proteases were designated as acid, neutral, and alkaline, depending upon their optimum pH range for casein digestion. Two of the proteases, the neutral and the alkaline, were rechromatographed.
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Z. Puhan (1969) studied this question.
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