February 5, 2013HypertensionOpen Access

Lack of Specificity of Commercial Antibodies Leads to Misidentification of Angiotensin Type-1 Receptor Protein

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Population

Human embryonic kidney cells expressing rat AT1A R fused with hemagglutinin tag at either amino terminus or…

Comparison

Immunoblotting with 3 distinct commercial AT1R… vs Antihemagglutinin antibody

Design

Preclinical

Authors

KEKatherine J. ElliottUniversity of South Florida KKKeita KimuraHiroshima Prefectural Hospital SESatoru EguchiVascular Medicine

Discussion

Member takes

Implication

Questions reliability of prior AT1R expression data from commercial antibodies; leaves open validated quantification methods in hypertension research.

Structured PICO

Population

Human embryonic kidney cells expressing rat AT1A R fused with hemagglutinin tag at either amino terminus or carboxyl terminus, and control cells

Intervention

Immunoblotting with 3 distinct commercial AT1R antibodies

Comparator

Antihemagglutinin antibody

Outcome

Detection of rat AT1A R protein (specificity of antibody binding)surrogate

Commercially available AT1R antibodies may lack the specificity required to accurately quantify receptor expression in basic science research.

Cite This Study

Elliott et al. (2013) studied this question.

synapsesocial.com/papers/6a90f3fe504d7cdc493e7ffe https://doi.org/10.1161/hypertensionaha.111.00943

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Implication

Questions reliability of prior AT1R expression data from commercial antibodies; leaves open validated quantification methods in hypertension research.

Lack of Specificity of Commercial Antibodies Leads to Misidentification of Angiotensin Type-1 Receptor Protein

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Lack of Specificity of Commercial Antibodies Leads to Misidentification of Angiotensin Type-1 Receptor Protein

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider