November 14, 2012HypertensionOpen Access

Lack of Specificity of Commercial Antibodies Leads to Misidentification of Angiotensin Type 1 Receptor Protein

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Population

Wild-type mice, AT knockout mice (ATKO), AT and AT double knockout mice (ATKO), and human embryonic kidney…

Design

Preclinical

Authors

MHMarcela HerreraTulane University MSMatthew A. SparksDeaconess Hospital AAAdolfo Alfonso-PecchioUniversity of North Carolina at Chapel Hill

Discussion

Member takes

Implication

Questions validity of AT1R studies using these antibodies; leaves open need for validated reagents in hypertension research.

Structured PICO

Population

Wild-type mice, AT(1A) knockout mice (AT(1A)KO), AT(1A) and AT(1B) double knockout mice (AT(1AB)KO), and human embryonic kidney cells overexpressing epitope-tagged AT(1A)R protein.

Intervention

Testing of 3 commonly used commercial putative AT(1)R antibodies using Western blotting and immunohistochemistry.

Outcome

Specificity of AT(1)R antibodies for detecting AT(1)R protein.surrogate

Commonly used commercial antibodies against the angiotensin II type 1 receptor exhibit nonspecific binding, which may lead to erroneous results in published studies.

Cite This Study

Herrera et al. (2012) studied this question.

synapsesocial.com/papers/6a856aa41238b1ee89d593fb https://doi.org/10.1161/hypertensionaha.112.203679

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Lack of Specificity of Commercial Antibodies Leads to Misidentification of Angiotensin Type 1 Receptor Protein

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Lack of Specificity of Commercial Antibodies Leads to Misidentification of Angiotensin Type 1 Receptor Protein

Discussion

Member takes

Implication

Structured PICO

Cite This Study

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