What question did this study set out to answer?

To determine the role of CCAAT/enhancer-binding protein (C/EBP) in regulating transcription of the phosphoenolpyruvate carboxykinase (PEPCK) gene promoter.

December 1, 1990Molecular and Cellular Biology

The Role of the CCAAT/Enhancer-Binding Protein in the Transcriptional Regulation of the Gene for Phosphoenolpyruvate Carboxykinase (GTP)

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Authors

EPEdwards A. ParkUniversity of Tennessee Health Science Center WRWilliam J. RoeslerUniversity of Saskatchewan JLJinsong LiuShenyang Ligong University

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Implication

In vitro molecular assays reveal that C/EBP transactivates the PEPCK promoter across multiple regulatory domains, indicating a key role in liver-specific metabolic gene control.

Key Points

To determine the role of CCAAT/enhancer-binding protein (C/EBP) in regulating transcription of the phosphoenolpyruvate carboxykinase (PEPCK) gene promoter.
Purification of rat liver nuclear proteins using a cyclic AMP-responsive element (CRE) oligonucleotide affinity column.
DNase I footprint analysis comparing recombinant C/EBP with purified 43-kDa liver nuclear protein.
Transient cotransfection assays using a C/EBP expression vector to evaluate PEPCK promoter transactivation.
Recombinant C/EBP generated a DNase I footprint identical to a 43-kDa rat liver nuclear protein, binding to CRE-1 and two liver-enriched upstream regulatory regions.
Cotransfection with C/EBP demonstrated robust transactivation of the PEPCK promoter through both the upstream liver-specific elements and the CRE.
The canonical CRE-binding protein (CREB) bound selectively to CRE-1 without interacting with the upstream C/EBP-binding sites.

Cite This Study

Park et al. (1990) studied this question.

synapsesocial.com/papers/6a90f686f69e67dea43612af https://doi.org/10.1128/mcb.10.12.6264-6272.1990

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Implication

In vitro molecular assays reveal that C/EBP transactivates the PEPCK promoter across multiple regulatory domains, indicating a key role in liver-specific metabolic gene control.

The Role of the CCAAT/Enhancer-Binding Protein in the Transcriptional Regulation of the Gene for Phosphoenolpyruvate Carboxykinase (GTP)

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Implication

Key Points

Cite This Study

The Role of the CCAAT/Enhancer-Binding Protein in the Transcriptional Regulation of the Gene for Phosphoenolpyruvate Carboxykinase (GTP)

Discussion

Member takes

Implication

Key Points

Cite This Study

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