Fertility and Motility of Bovine Spermatozoa in Buffered Whole Egg Extenders

In previous reports from this laboratory, Dunn and Bratton (2, 3) presented preliminary data showing that satisfactory motility and fertility of bovine spermatozoa could be obtained with a citrate-sulfasuxidine buffered liquid whole egg extender.The present paper reports results of more extensive investigations on the fertility and motility of bovine spermatozoa in this type of extender.EXPERIMENTAL Two field trials were conducted, the first from December 28, 1948, to January 11, 1949, and the second from May 16 to June 9, 1949.In the first trial each of 69 ejaculates from 37 Holstein bulls was split two ways.One portion was extended in 2.9 per cent citrate-sulfanilamide-yolk (2.9 CSAY) and the other in 1.9 per cent citrate-sulfasuxidine-whole egg (1.9 CSSWE).In the second trial each of 145 ejaculates from 48 bulls, 31 Holsteins and 17 Guernseys, was divided into four portions and each portion extended in one of the following extenders.3.5 CSAY, 2.9 CSAY, 2.9 CSSWE, and 1.9 CSSWE.The composition of the buffers and extenders are shown in table 1.The buffers were prepared once a week and the extenders during the afternoon of the day prior to their use.