June 1, 2003Journal of Biological ChemistryOpen Access

ATP Binding Modulates the Nucleic Acid Affinity of Hepatitis C Virus Helicase

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Population

Hepatitis C virus (HCV) helicase (in vitro biochemical model)

Comparison

ATP analogs vs Absence of ATP or presence of ADP

Design

Preclinical

Authors

MLMikhail K. LevinBank of America MGMadhura M. Gurjar SPSmita S. PatelRutgers, The State University of New Jersey

Discussion

Member takes

Implication

Hypothesis-generating for HCV helicase mechanism; leaves open translation to antiviral strategies.

Structured PICO

Population

Hepatitis C virus (HCV) helicase (in vitro biochemical model)

Intervention

ATP analogs (beta-gamma methylene/amine analogs of ATP, ATPgammaS, [AlF4]ADP, [BeF3]ADP)

Comparator

Absence of ATP or presence of ADP

Outcome

Nucleic acid binding affinity and ATPase activity inhibitionsurrogate

The nucleic acid binding site of HCV helicase is allosterically modulated by the ATPase reaction, supporting a Brownian motor model for unidirectional translocation.

Cite This Study

Levin et al. (2003) studied this question.

synapsesocial.com/papers/6a91126b0d6df1a827c1bbce https://doi.org/10.1074/jbc.m301283200

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ATP Binding Modulates the Nucleic Acid Affinity of Hepatitis C Virus Helicase

Discussion

Member takes

Implication

Structured PICO

Cite This Study

ATP Binding Modulates the Nucleic Acid Affinity of Hepatitis C Virus Helicase

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider