June 10, 2003Journal of Cellular Physiology

Phenotype modulation in cultures of vascular smooth muscle cells from diabetic rats: Association with increased nitric oxide synthase expression and superoxide anion generation

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Structured PICO

Population

Cultures of vascular smooth muscle cells (vSMC) obtained from aorta medial layer of 10 diabetic (90% pancreatectomy) and 10 control (sham surgery) rats (n=20)

Intervention

Diabetes induced by 90% pancreatectomy

Comparator

Sham surgery (control)

Outcome

Cell morphology, proliferative response to serum, alpha-SMactin levels, eNOS expression and activity, cGMP intracellular content, and superoxide anion releasesurrogate

In a diabetic rat model, vascular smooth muscle cells exhibit a proliferative phenotype with increased eNOS expression but reduced NO bioavailability due to concurrent superoxide anion overproduction.

Discussion

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Implication

Highlights oxidative stress as a driver of diabetic vascular SMC changes in rats; hypothesis-generating and should not yet change practice.

Cite This Study

Pandolfi et al. (2003) studied this question.

synapsesocial.com/papers/6a911bb1504d7cdc493e8d74 https://doi.org/10.1002/jcp.10325

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Implication

Highlights oxidative stress as a driver of diabetic vascular SMC changes in rats; hypothesis-generating and should not yet change practice.

Phenotype modulation in cultures of vascular smooth muscle cells from diabetic rats: Association with increased nitric oxide synthase expression and superoxide anion generation

Structured PICO

Discussion

Implication

Cite This Study

Phenotype modulation in cultures of vascular smooth muscle cells from diabetic rats: Association with increased nitric oxide synthase expression and superoxide anion generation

Structured PICO

Discussion

Implication

Cite This Study

Also Consider

Also Consider