Viral infection or puromycin treatment does not inhibit DNA repair replication in HeLa cells despite severely inhibiting overall cellular DNA synthesis.
Repair replication proceeds independently of inhibited bulk synthesis; leaves open relevance to viral pathogenesis or in vivo recovery.
We examined repair replication of HeLa cell deoxyribonucleic acid (DNA) in cells infected with mengovirus or Newcastle disease virus or treated with puromycin. Cellular DNA was damaged by ultraviolet light and then pulse-labeled with (3)H-thymidine. Autoradiographic analysis of non-S-phase DNA synthesis (repair replication) showed that there was no inhibition of this process at a time when overall cellular DNA synthesis was severely inhibited by either virus infection or puromycin treatment.
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Hand et al. (1971) studied this question.
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