Key result
Nonproductive human CMV infection of guinea pig embryo cells induced cellular RNA and DNA synthesis, whereas heat- and UV-inactivated virus failed to induce synthesis.
Why the study?
Does nonproductive human CMV infection stimulate macromolecular synthesis in guinea pig embryo cells?
Does nonproductive human CMV infection stimulate macromolecular synthesis in guinea pig embryo cells?
Nonproductive human CMV infection stimulates cellular RNA and DNA synthesis in guinea pig embryo cells, requiring active virus.
Answers are grounded in this paper and cite their sources.
Active virus required for induction in guinea pig cells; leaves open relevance to human CMV pathogenesis.
Nonproductive human CMV infection of G.P. embryo cells induced cellular RNA and DNA synthesis. RNA species synthesized in abortively infected G.P. cells included ribosomal 28S, 18S, and 4S transfer RNA. Virus inactivated with heat and UV light failed to induce cellular RNA and DNA synthesis. Evidence for increased DNA and RNA synthesis in infected cells was provided by studies of:(a) incorporation of thymidine and uridine, (b) extraction and separation of cellular DNA and RNA on sedimentation gradients, (c) autoradiography, and (d) measurement of mitotic index.
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Furukawa et al. (1975) studied Nonproductive human cytomegalovirus (CMV) infection. Human CMV infection vs. Heat and UV light inactivated virus was evaluated on Cellular RNA and DNA synthesis. Nonproductive human CMV infection of guinea pig embryo cells induced cellular RNA and DNA synthesis, whereas heat- and UV-inactivated virus failed to induce synthesis.
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