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A rapid procedure is described for the direct assay of limonin which makes use of a new detecting system and a new solvent system for thin‐layer chromatography (t.l.c.) of limonoids. The detecting system, which involves exposure to bromine vapour and spraying with Tollens' reagent, is more selective and sensitive than previous methods, detecting five limonoids in orange juice extracts, whereas previous t.l.c. procedures only gave one spot.
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B. V. Chandler (1971) studied this question.
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