The effect of dietary oleic and palmitic acids on the composition and turnover rates of liver phospholipins

Ozaki (1927) found that rats fed on diets containing 20 % oleic acid gained weight, whereas rats on diets * When choline supplements were given the casein was sprayed with a solution of choline chloride to give 30 mg.choline/lO g. diet.t NaCl, 22 g.; CaHP04.H20, 130 g.; potassium citrate, 125 g.; MgSO4.7H20, 30 g.; ferric citrate, 5 g.; 0-7 g. of trace mixture (KI, 12 g.; NaF, 10 g.; MnSO4, 2 g.CU2I,2 1 g.; KAJ(S04)2, 1g.; ZnSO4.7H20, I g. de Loureiro, 1931).t Grade A, 100 mesh 'solka floc'; a highly purified cellulose powder obtainable from Johnsen, Jorgensen and Wettre Ltd., London.Animals were starved for 24 hr.and then placed on the diets for 12 days.The palmitic acid diet was not eaten so readily as the oleic acid diet and therefore approximate pair feeding between litter mates was adopted.Controls were fed ad lib. on a stock diet consisting ofskimmed milk powder, 20%; ground yellow maize, 18%; rolled oats, 15%; barley meal, 15%; biscuit meal, 15%; wheat germ, 5%; palm kerneloil, 5 %; dried yeast, 5 % and bran, 2 %.Each animal received 10 uc.Of 32p as Na2H32PO4 in distilled water by intraperitoneal injection, and was killed by decapitation 4 hr.later.In order to reduce the effect of recently ingested fat on the specific activities of liver phospholipins, food and waterwereremovedfromtheanimals4hr.beforetheinjection of 82p.(Specific activity (SA) =% injected 32P/mg.phosphorus.) 1.