Summary It has been shown that glycerol-1-C 14 enters bull spermatozoa in measurable amounts and is converted to C 14 O 2 during anaerobic incubation. The oxidation of glycerol was shown to be due to the metabolic processes of the spermatozoa and not to enzymatic activity of seminal plasma. Incubation of sperm, which had been separated from the seminal plasma, in the presence of glycerol-1-C 14 showed high radioactivity in the CO 2 given off, whereas there was relatively no activity present in the CO 2 collected from seminal plasma incubated with glycerol-1-C 14 . Bull spermatozoa which had been separated from the seminal plasma showed a higher uptake and a higher metabolic utilization of glycerol than did spermatozoa in the presence of seminal plasma.
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O’Dell et al. (1956) studied this question.
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