November 1, 1989Blood
Inhibition of chemotaxis Ng-monomethyl-L-arginine: a role for cyclic GMP
Why the study?
Does NG-monomethyl-L-arginine (NMA) inhibit chemotaxis and microbial killing in human peripheral blood neutrophils?
Population
Human peripheral blood neutrophils
Comparison
NG-monomethyl-L-arginine with or without… vs Cells incubated without NMA
Design
Preclinical
Authors
Discussion
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Implication
NMA effects warrant caution in experimental models; hypothesis-generating for selective NO modulation of human neutrophil chemotaxis.
Structured PICO
Does NG-monomethyl-L-arginine (NMA) inhibit chemotaxis and microbial killing in human peripheral blood neutrophils?
P
Population
Human peripheral blood neutrophils
I
Intervention
NG-monomethyl-L-arginine (NMA) with or without additional L-arginine or dibutyryl cyclic guanosine monophosphate (cGMP)
C
Comparator
Cells incubated without NMA
O
Outcome
Chemotaxis (evaluated using a 48-well micro-Boyden chamber) and microbial killing (evaluated using S aureus strains D2C and 502A)surrogate
The L-arginine metabolism to NO and its effect on cGMP levels are important for neutrophil chemotaxis but not for microbial killing of S. aureus.
Cite This Study
Kaplan et al. (1989) studied this question.