Key result
Treatment of human endothelial cells with inflammatory cytokines TNF-α and IFN-γ synergistically decreased CAR protein expression by up to 99% and reduced adenovirus binding by 80%.
p-value: p=<0.001
Inflammatory cytokines downregulate CAR expression in endothelial cells, reducing adenovirus binding and gene transfer efficiency.
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May limit adenoviral gene delivery in inflamed endothelium; leaves open whether cytokine blockade could restore CAR and vector efficacy.
Vincent et al. (2004) studied this question. Tumor necrosis factor alpha (TNF-α) and gamma interferon (IFN-γ) vs. Untreated cells was evaluated on Coxsackievirus-adenovirus receptor (CAR) protein expression and adenovirus binding (p=<0.001). Treatment of human endothelial cells with inflammatory cytokines TNF-α and IFN-γ synergistically decreased CAR protein expression by up to 99% and reduced adenovirus binding by 80%.
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