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SUMMARY Aerosol-inactivation of bacteriophage T1 is prevented by peptone and by apolar amino acids (leucine, phenylalanine). The protecting concentration is related to the salt concentration in the spray-medium, which determines the amino acid concentration in the aerosol particle after evaporation to equilibrium. The protective action of surface active agents supports the hypothesis that inactivation is due to surface inactivation. The surface occupation of the protecting amino acids was calculated with the Gibbs adsorption formula and agrees with amounts necessary to cover the air/water interface, thus preventing the phage from reaching the surface. The decrease of amino acid concentration in the aerosol particle by surface adsorption is calculated in the appendix.
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Trouwborst et al. (1972) studied this question.
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