Key result
Treatment of human umbilical vein endothelial cells with L-arginine, L-citrulline, Vitamin D3, and antioxidants synergistically increased the cytoprotective [NO]/[ONOO−] ratio by 50%.
Why the study?
Deficiency in bioavailable nitric oxide is linked to endothelial NO synthase dysfunction, oxidative stress, and cardiovascular diseases, motivating evaluation of treatments that restore this balance.
Does a supplement containing L-arginine, L-citrulline, Vitamin D3, and antioxidants improve the [NO]/[ONOO−] ratio in human umbilical vein endothelial cells?
Does a supplement containing L-arginine, L-citrulline, Vitamin D3, and antioxidants improve the [NO]/[ONOO−] ratio in human umbilical vein endothelial cells?
A combination of L-arginine, L-citrulline, Vitamin D3, and antioxidants synergistically enhances nitric oxide bioavailability and reduces oxidative stress in human endothelial cells in vitro.
Endothelial cell synergy is hypothesis-generating; leaves open clinical translation in cardiovascular disease.
Pharmacognosy Research,2020,12,1,17-23.DOI:10.4103/pr.pr_79_19Published:February 2020Type:Original ArticleAuthors:Hazem Dawoud, and Tadeusz Malinski Author(s) affiliations:Hazem Dawoud, Tadeusz Malinski Department of Chemistry and Biochemistry, Nanomedical Research Laboratories, Ohio University, Athens, Ohio, USA Abstract:Background: Nitric oxide (NO) is a crucial signaling molecule which regulates the blood flow and prevents the adhesion of blood components to the vascular wall. A deficiency in bioavailable NO concentration is associated with the dysfunction of endothelial NO synthase (eNOS) and/or an increase in oxidative stress. The deficiency of bioavailable NO is a common denominator of several cardiovascular diseases, including diabetes, atherosclerosis, and hypertension. Materials and Methods: We used a nanomedical technology to elucidate the balance between bioavailable NO and oxidative stress (peroxynitrite ONOO−) in human umbilical vein endothelial cells (HUVECs) treated with a supplement containing L‑arginine, L‑citrulline, Vitamin D3, and antioxidants. Nanosensors, with a diameter of 200–300 nm, are capable of measuring in situ NO and peroxynitrite (ONOO−) concentrations produced by single endothelial cells. Results: The ratio of the concentration of cytoprotective NO [NO] to the concentration of cytotoxic peroxynitrite [ONOO−] was used to estimate the efficiency of eNOS. HUVECs incubated with L‑citrulline, L‑arginine, and Vitamin D3 increased the [NO]/[ONOO−] ratio by 25%, while in the presence of antioxidants, the increase was 15%. The synergistic effect between the mix of L‑arginine, L‑citrulline, Vitamin D3, and antioxidants was a favorable increase of the overall [NO]/[ONOO−] ratio by 50%. Conclusion: The findings of the study presented here clearly indicate that L‑arginine, L‑citrulline, and Vitamin D3 can significantly alter the function of the endothelium and NO production, in a favorable manner, while pointedly reducing ONOO− – the main component of oxidative stress. This effect can be significantly potentiated in the presence of antioxidants. Keywords:Antioxidant, Endothelium, L‑arginine, L‑citrulline, Nitric oxide, Peroxynitrite, Vitamin D3View:PDF (1.26 MB)
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Maliñski et al. (2020) studied this question. L-arginine, L-citrulline, Vitamin D3, and antioxidants was evaluated on [NO]/[ONOO−] ratio. Treatment of human umbilical vein endothelial cells with L-arginine, L-citrulline, Vitamin D3, and antioxidants synergistically increased the cytoprotective [NO]/[ONOO−] ratio by 50%.
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