What question did this study set out to answer?

To determine whether hypertrophic chondrocytes release aggrecan-degrading lysosomal hydrolases into growth plate cartilage via calcium-dependent lysosomal exocytosis during endochondral bone formation.

February 10, 2012Biology OpenOpen Access

Evidence for lysosomal exocytosis and release of aggrecan-degrading hydrolases from hypertrophic chondrocytes, in vitro and in vivo

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Authors

EBEdward BastowThe University of Western Australia KLKarena LastThe University of Melbourne SGS. Ya. GolubRoyal Children's Hospital

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Implication

Experimental study demonstrates calcium-dependent lysosomal exocytosis of cartilage-degrading hydrolases from hypertrophic chondrocytes, highlighting an alternative pathway in bone development.

Key Points

To determine whether hypertrophic chondrocytes release aggrecan-degrading lysosomal hydrolases into growth plate cartilage via calcium-dependent lysosomal exocytosis during endochondral bone formation.
Treated cultured epiphyseal chondrocytes in vitro with the calcium ionophore ionomycin to assess cell-surface LAMP1 exposure and secretion of cathepsin D, β-hexosaminidase, and aggrecan- and hyaluronan-degrading activity.
Examined the in vivo distribution and colocalization of SNARE proteins (VAMP-8 and VAMP7) with LAMP1 at the cell surface within secondary ossification centers in developing mouse tibiae.
Ionomycin treatment induced cell-surface translocation of LAMP1 and stimulated the release of lysosomal enzymes cathepsin D and β-hexosaminidase, alongside aggrecan- and hyaluronan-degrading activity in vitro.
VAMP-8 and VAMP7 colocalized with LAMP1 at the cell surface in mouse tibial secondary ossification centers, demonstrating active lysosomal exocytosis during growth plate cartilage resorption in vivo.

Cite This Study

Bastow et al. (2012) studied this question.

synapsesocial.com/papers/6a916178388f32339cd2cdf6 https://doi.org/10.1242/bio.2012547

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Implication

Experimental study demonstrates calcium-dependent lysosomal exocytosis of cartilage-degrading hydrolases from hypertrophic chondrocytes, highlighting an alternative pathway in bone development.

Evidence for lysosomal exocytosis and release of aggrecan-degrading hydrolases from hypertrophic chondrocytes, in vitro and in vivo

Discussion

Member takes

Implication

Key Points

Cite This Study

Evidence for lysosomal exocytosis and release of aggrecan-degrading hydrolases from hypertrophic chondrocytes, in vitro and in vivo

Discussion

Member takes

Implication

Key Points

Cite This Study

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