Key result
Pretreatment of MA104 cells with neuraminidase reduced the infectivity of four avian rotavirus strains to 61-83% of pretreatment levels, suggesting they utilize sialic acid as receptors.
Avian rotaviruses primarily utilize sialic acid-containing molecules as receptors on MA104 cells, as demonstrated by reduced infectivity following neuraminidase treatment.
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Supports sialic acid receptor use in MA104 cells; leaves open in vivo relevance and therapeutic translation for avian rotaviruses.
To determine the characters of receptors on target cells for avian rotaviruses, the receptors on MA104 cells for the pigeon rotavirus PO-13, the turkey rotaviruses Ty-1 and Ty-3, and the chicken rotavirus Ch-1 were analyzed. Pretreatment of MA104 cells with neuraminidase greatly reduced the infection by all of the four avian rotavirus strains. Binding of the cell-attachment protein, purified VP8 expressed in bacteria, of strain PO-13 to MA104 cells was also inhibited by pretreatment of cells with neuraminidase. These findings suggest that avian rotaviruses primarily utilize sialic acid-containing molecules as receptors on MA 104 cells.
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Sugiyama et al. (2004) studied Avian rotavirus infection. Neuraminidase vs. Untreated control cells was evaluated on Infectivity titers (percentage of control). Pretreatment of MA104 cells with neuraminidase reduced the infectivity of four avian rotavirus strains to 61-83% of pretreatment levels, suggesting they utilize sialic acid as receptors.
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