Key result
Alamandine co-therapy significantly reduced doxorubicin-induced nephrotoxicity in rats, decreasing serum creatinine from 1.61 to 1.06 mg/dl (p<0.01) compared to doxorubicin alone.
Why the study?
This study aimed to evaluate the protective effects of alamandine against doxorubicin-induced nephrotoxicity in rats.
Does alamandine prevent doxorubicin-induced nephrotoxicity in rats?
Does alamandine prevent doxorubicin-induced nephrotoxicity in rats?
Absolute Event Rate: 1.06% vs 1.61%
p-value: p=<0.01
Alamandine co-administration prevents doxorubicin-induced nephrotoxicity in a rat model by reducing inflammation and oxidative stress.
Alamandine mitigated DOX nephrotoxicity in rats; hypothesis-generating and requires human trials before any clinical consideration.
BACKGROUND: This study aimed to evaluate the protective effects of alamandine, a new member of the angiotensin family, against doxorubicin (DOX)-induced nephrotoxicity in rats. METHODS: Rats were intraperitoneally injected with DOX (3.750 mg/kg/week) to reach a total cumulative dose of 15 mg/kg by day 35. Alamandine (50 µg/kg/day) was administered to the rats via mini-osmotic pumps for 42 days. At the end of the experiment, rats were placed in the metabolic cages for 24 h so that their water intake and urine output could be measured. After scarification, the rats' serum and kidney tissues were collected, and biochemical, histopathological, and immunohistochemical studies were carried out. RESULTS: DOX administration yielded increases in pro-inflammatory cytokines, including interleukin (IL)-1β and IL-6, pro-fibrotic proteins transforming growth factor-β (TGF-β), pro-inflammatory transcription factor nuclear kappa B (NF-κB), kidney malondialdehyde (MDA), creatinine clearance, blood urea nitrogen (BUN), and water intake. On the other hand, the DOX-treated group exhibited decreased renal superoxide dismutase (SOD), renal glutathione peroxidase (GPx) activity, and urinary output. Alamandine co-therapy decreased these effects, as confirmed by histopathology and immunohistochemical analysis. CONCLUSIONS: The results suggest that alamandine can prevent nephrotoxicity induced by DOX in rats.
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Hekmat et al. (2021) studied Doxorubicin-induced nephrotoxicity (n=35). Alamandine vs. Doxorubicin alone was evaluated on Serum creatinine (mg/dl) (p=<0.01). Alamandine co-therapy significantly reduced doxorubicin-induced nephrotoxicity in rats, decreasing serum creatinine from 1.61 to 1.06 mg/dl (p<0.01) compared to doxorubicin alone.
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