What question did this study set out to answer?

To determine whether human platelet-derived growth factor alone can trigger amino acid transport and protein synthesis in quiescent human diploid fibroblasts in the absence of plasma components.

May 1, 1982Proceedings of the National Academy of SciencesOpen Access

Human platelet-derived growth factor stimulates amino acid transport and protein synthesis by human diploid fibroblasts in plasma-free media.

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To determine whether human platelet-derived growth factor alone can trigger amino acid transport and protein synthesis in quiescent human diploid fibroblasts in the absence of plasma components.
Exposed quiescent human diploid fibroblasts to purified human platelet-derived growth factor (PDGF) in plasma-free culture media.
Measured system A amino acid uptake, acid-insoluble [3H]leucine incorporation into protein, and the inhibitory effects of specific PDGF antiserum.
PDGF induced an increase in system A amino acid uptake that required a 45-minute exposure for maximal effect and depended upon ongoing protein synthesis.
PDGF alone in plasma-free conditions significantly stimulated both amino acid transport and [3H]leucine incorporation, effects that were completely blocked by anti-PDGF antiserum.

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Implication

In vitro study demonstrates that platelet-derived growth factor independently stimulates amino acid uptake and protein synthesis in human fibroblasts, indicating direct early metabolic regulation.

Cite This Study

Owen et al. (1982) studied this question.

synapsesocial.com/papers/6a91752735eca4d44dab5f43 https://doi.org/10.1073/pnas.79.10.3203

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Implication

In vitro study demonstrates that platelet-derived growth factor independently stimulates amino acid uptake and protein synthesis in human fibroblasts, indicating direct early metabolic regulation.

Human platelet-derived growth factor stimulates amino acid transport and protein synthesis by human diploid fibroblasts in plasma-free media.

Key Points

Discussion

Implication

Cite This Study

Human platelet-derived growth factor stimulates amino acid transport and protein synthesis by human diploid fibroblasts in plasma-free media.

Key Points

Discussion

Implication

Cite This Study

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