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The analysis of steady state mRNA levels in cells or tissues that are undergoing growth and developmental changes can be problematic due to the lack of invariant control genes.Direct staining of agarose gels for 18s and 28s ribosomal RNAs is often used to control for loading errors.This approach does not control for transfer and hybridization artifacts.Several genes including actin, GAPDH, and B2 microglobulin have been used successfully as control genes.However, these genes are not always suitable controls when growth state is changing or limited
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Levine et al. (1993) studied this question.
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