Key result
TTX-sensitive voltage-gated Na+ channels are present in mouse and rat mesenteric artery smooth muscle cells, but their detection depends critically on the enzymatic dissociation conditions used.
Population
Smooth muscle cells (SMCs) from mouse and rat mesenteric arteries
Comparison
Enzymatic dissociation using collagenase and… vs Enzymatic dissociation using papain and…
Design
Preclinical
Authors
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Method-dependent detection warrants caution interpreting vascular myocyte electrophysiology; leaves open channels' role in intact resistance arteries.
The study demonstrates that TTX-sensitive voltage-gated Na+ channels are present in mesenteric artery smooth muscle cells, and their detection is critically dependent on the enzymatic dissociation method used.
Berra‐Romani et al. (2005) studied this question. Enzymatic dissociation protocols was evaluated on Presence and properties of voltage-gated Na+ channels. TTX-sensitive voltage-gated Na+ channels are present in mouse and rat mesenteric artery smooth muscle cells, but their detection depends critically on the enzymatic dissociation conditions used.
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