Isolation of Adult Cardiomyocytes Initiates a Return of Inositol Trisphosphate Phosphorylating Activity

We have previously reported that the addition of noradrenaline to [3H]-inositol-labelled adult rat atria or isolated perfused hearts caused the release of inositol-1,4,5-trisphosphate, which was metabolized by dephosphorylation to inositol-4-monophosphate. Inositol-1,3,4,5-tetrakisphosphate and its dephosphorylation products were not detected. 2. In the current study, the addition of noradrenaline to [3H]-inositol-labelled adult rat cardiomyocytes caused the release of inositol-1,4,5-trisphosphate, which was metabolized in part by phosphorylation to inositol-1,3,4,5-tetrakisphosphate. 3. These results demonstrate that the isolation and culture of rat adult cardiomyocytes initiates enhanced generation of inositol-1,3,4,5-tetrakisphosphate. This change would be expected to enhance the calcium response of the cells to stimulation of alpha 1-adrenoceptors.