Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
June 27, 2012Cardiovascular Research

Disease characterization using LQTS-specific induced pluripotent stem cells

View Full Paper
Ask AI
Bookmark
Share

Key result

LQTS-specific iPSC-derived cardiomyocytes exhibited prolonged field potential duration and severe arrhythmias upon IKr blockade, revealing a dominant-negative IKs channel trafficking deficiency.

Population

Somatic cells from a patient with sporadic Long QT syndrome and controls, reprogrammed into induced…

Comparison

Electrophysiological analysis using a… vs Control iPSC-derived cardiomyocytes.

Design

Preclinical

Authors

TEToru EgashiraTokyo Metropolitan Tama Medical CenterSYShinsuke YuasaHeart Failure & TransplantTSTomoyuki SuzukiInstitute of Livestock and Grassland Science

Discussion

Loading...

Member takes

Overview

iPSC models may characterize LQTS mutations and drug responses; leaves open clinical translation pending in vivo validation.

Structured PICO

P
Population
In vitro study using induced pluripotent stem cells (iPSCs) derived from a patient with sporadic LQTS and controls to characterize ion channel cardiac disorders.
E
Exposure
Electrophysiological analysis using a multi-electrode array (MEA) system and drug testing (IKr blocker E4031, IKs blocker chromanol 293B).
C
Comparator
Control iPSC-derived cardiomyocytes.
O
Outcome
Field potential duration (FPD) and electrophysiological response to ion channel blockers.surrogate

Patient-specific iPSCs can successfully model sporadic Long QT syndrome and characterize novel mutations and drug responses in vitro.

Cite This Study

Egashira et al. (2012) studied Long QT syndrome (LQTS). LQTS-specific induced pluripotent stem cells (iPSCs) vs. Control iPSCs was evaluated on Field potential duration (FPD) and response to ion channel blockers. LQTS-specific iPSC-derived cardiomyocytes exhibited prolonged field potential duration and severe arrhythmias upon IKr blockade, revealing a dominant-negative IKs channel trafficking deficiency.

synapsesocial.com/papers/6a918508dc264a2e5a1201aehttps://doi.org/10.1093/cvr/cvs206
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Extracellular K + Is a Prerequisite for the Function and Plasma Membrane Stability of HERG Channels2010 · 47 citations
  2. 2Treating the Long-QT Syndrome in the Era of Implantable Defibrillators2009 · 23 citations
  3. 3Slow Delayed Rectifier Potassium Current (IKs) and the Repolarization Reserve2007 · 88 citations
  4. 4Sudden Death Due to Cardiac Arrhythmias2001 · 1,477 citations
  5. 5Genetic Testing in the Long QT Syndrome2005 · 471 citations