The reactions of diazonium compounds with amino acids and proteins

A PAPER FLUORIMETRIC METHOD 651 potassium tert.-butoxide in tert.-butanol on a A4-3-oxo steroid.This solution method may be more convenient for measuring relatively pure steroids.The paper method, however, has the advantage that additional separation of steroids is obtained during the analytical procedure and the presence of com- pounds, other than the one to be measured, is revealed; this method may therefore be preferable for the analysis of impure fractions.It seems likely that the method for measuring fluorescent compounds on paper can be applied to substances other than A4-3-oxo steroids.During the preparation of this paper our attention was draw-n to the description of such an application by other workers (Mourodineanu, Sanford & Hitchcock, 1955).SUMMARY 1.A method has been described in which amounts