Why the study?
Does transgenic expression of FMDV-specific shRNA reduce susceptibility to FMDV infection in pigs?
Does transgenic expression of FMDV-specific shRNA reduce susceptibility to FMDV infection in pigs?
Transgenic expression of FMDV-specific shRNA provides a viable strategy for producing pigs with enhanced resistance to foot-and-mouth disease virus.
Supports RNAi transgenesis for FMD resistance in pigs; leaves open scalability, biosafety, and regulatory translation to livestock.
Foot-and-mouth disease virus (FMDV) is an economically devastating viral disease leading to a substantial loss to the swine industry worldwide. A novel alternative strategy is to develop pigs that are genetically resistant to infection. Here, we produce transgenic (TG) pigs that constitutively expressed FMDV-specific short interfering RNA (siRNA) derived from small hairpin RNA (shRNA). In vitro challenge of TG fibroblasts showed the shRNA suppressed viral growth. TG and non-TG pigs were challenged by intramuscular injection with 100 LD50 of FMDV. High fever, severe clinical signs of foot-and-mouth disease and typical histopathological changes were observed in all of the non-TG pigs but in none of the high-siRNA pigs. Our results show that TG shRNA can provide a viable tool for producing animals with enhanced resistance to FMDV.
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Hu et al. (2015) studied this question.
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