An Alkali Resistant Thermostable Hemopoietic Factor in Liver

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Article1 January 1963An Alkali Resistant Thermostable Hemopoietic Factor in LiverCARROLL M. LEEVY, M.D., F.A.C.P., HERMAN BAKER, PH.D., OSCAR FRANK, PH.D.CARROLL M. LEEVY, M.D., F.A.C.P.Search for more papers by this author, HERMAN BAKER, PH.D.Search for more papers by this author, OSCAR FRANK, PH.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-58-1-37 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptExistence in the liver of hemopoietic principles, as yet undefined, is suggested by the occasional response to crude liver extract of anemia refractory to commercial preparations of vitamin B12 and folic acid. Such factors may also account for sustained remissions induced in pernicious anemia by oral liver extract (1), since such remissions cannot be attributed to either the crystalline B12 or folic acid content of the extract.Alkaline hydrolysis destroys crystalline B12, folic acid, and their known analogues; however, liver extract subjected to this procedure still supports the growth of microorganisms used for the clinical assay of these vitamins (2,...References1. MINOTMURPHYFITZMONROE GRWPRRD: Observations on patients with pernicious anemia partaking of a special diet. Trans. Ass. Amer. Physicians 41: 72, 1926. Google Scholar2. LEEVYBAKERFRANKZIEFER CMHOH: A metabolic substitute for vitamin B12. J. Clin. Invest. 40: 1057, 1961. Google Scholar3. BAKERFRANKLEEVY HOCM: An alkali resistant factor with B12 activity for protozoa and man. Experientia 18: 458, 1962. CrossrefMedlineGoogle Scholar4. DUTTA SK: Studies on growth principles in liver. I. 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MARSHALLJANDL RAJH: Responses in "physiologic" doses in folic acid in the megaloblastic anemias. Arch. Intern. Med. 105: 352, 1960. CrossrefMedlineGoogle Scholar24. GORDON AS: Hemopoietine. Physiol. Rev. 39: 1, 1959. CrossrefMedlineGoogle Scholar25. TOOHEYBARKER JIHA: Isolation of coenzyme B12 from liver. J. Biol. Chem. 236: 560, 1961. CrossrefMedlineGoogle Scholar26. RABINOWITZ JC: Folic acid, in The Enzymes, vol. 2, ed. by Boyer, P. D., Lardy, H., and Myrback, K., Academic Press, New York. 1960, pp. 185, 196. Google Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: From the Division of Hepatic Metabolism and Nutrition, Department of Medicine, Seton Hall College of Medicine and Dentistry and Jersey City Medical Center, Jersey City, New Jersey.Presented at the Forty-third Annual Session of The American College of Physicians, Philadelphia, Pennsylvania, April 9-13, 1962.Supported in part by National Institutes of Health grants A-4437 (C1), C-4956 (C2), H-4530 (C2), Clinical Research Center grant OG-17R1, and a grant from the National Vitamin Foundation.Requests for reprints should be addressed to Carroll M. Leevy, M.D., Division of Hepatic Metabolism and Nutrition, Department of Medicine, Seton Hall College of Medicine and Dentistry, Jersey City 4, New Jersey. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited ByRole of 5′-Deoxy-5′-Methylthioadenosine in growth of several microbial B12 requirersVariations in the Growth Response of Four Different Vitamin B 12 Assay Microorganisms to the Same Tissue and Standard PreparationsClinical Aspects of the Hepatic CirculationThe Liver and its DiseasesMetabolic and Nutritional Effects of Alcoholism 1 January 1963Volume 58, Issue 1Page: 37-46KeywordsAnemiaAttentionDentistryFolic acidHematology and oncologyLactobacillusLiverNutritionPernicious anemiaResearch grants Issue Published: 1 January 1963 PDF DownloadLoading ...