February 1, 1971The Journal of Cell BiologyOpen Access

Observations on Organization of Z-Disk Components and on Rod-Bodies of Z-Disk Origin

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Population

Frog, rat, and human muscle specimens

Comparison

Fixation in aldehyde + osmium tetroxide… vs Fixation in osmium tetroxide (O) alone

Design

Preclinical

Authors

RMRonald D. MacdonaldCanadian Animal Health Institute AEAndrew G. EngelBroad Institute

Discussion

Member takes

Overview

Fixation method alters Z-disk appearance across species; leaves open standardized protocols for reliable ultrastructural comparisons in muscle research.

Structured PICO

Population

Frog, rat, and human muscle specimens

Intervention

Fixation in aldehyde + osmium tetroxide, aldehyde alone, and low-ionic-strength extraction (LISE) prior to fixation

Comparator

Fixation in osmium tetroxide (O) alone

Outcome

Z-disk morphology (Z-1 vs Z-2 pattern) and rodlike structuressurrogate

The study demonstrates that Z-disk morphological patterns in muscle vary depending on the chemical fixatives used, highlighting the importance of fixation methods in ultrastructural studies.

Cite This Study

Macdonald et al. (1971) studied this question.

synapsesocial.com/papers/6a9285c5fa60aefc093ab003 https://doi.org/10.1083/jcb.48.2.431

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February 1, 1971The Journal of Cell BiologyOpen Access

Observations on Organization of Z-Disk Components and on Rod-Bodies of Z-Disk Origin

View Full Paper

Ask AI

Bookmark

Population

Frog, rat, and human muscle specimens

Comparison

Fixation in aldehyde + osmium tetroxide… vs Fixation in osmium tetroxide (O) alone

Design

Preclinical

Authors

RMRonald D. MacdonaldCanadian Animal Health Institute AEAndrew G. EngelBroad Institute

Discussion

Member takes

Overview

Fixation method alters Z-disk appearance across species; leaves open standardized protocols for reliable ultrastructural comparisons in muscle research.

Structured PICO

Population

Frog, rat, and human muscle specimens

Intervention

Fixation in aldehyde + osmium tetroxide, aldehyde alone, and low-ionic-strength extraction (LISE) prior to fixation

Comparator

Fixation in osmium tetroxide (O) alone

Outcome

Z-disk morphology (Z-1 vs Z-2 pattern) and rodlike structuressurrogate

The study demonstrates that Z-disk morphological patterns in muscle vary depending on the chemical fixatives used, highlighting the importance of fixation methods in ultrastructural studies.

Cite This Study

Macdonald et al. (1971) studied this question.

synapsesocial.com/papers/6a9285c5fa60aefc093ab003 https://doi.org/10.1083/jcb.48.2.431

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

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