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SUMMARY Non-nodulating mutants ofRhizobium trifoliiwere found in cultures incubated at 35 °C for 7 days. From among ten strains incubated at the elevated temperature six became non-nodulating. The frequency of Nod−mutants inR. trifoliicultures varied from 1 to 75% depending on the strain tested. It is suggested that gene(s) controlling nodulation are located on a large plasmid.
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Zurkowski et al. (1978) studied this question.
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